Anti-Rat CXCL2 (Clone 123802)

Anti-Rat CXCL2 (Clone 123802)

Product No.: C1433

[product_table name="All Top" skus="C1433"]

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Clone
123802
Target
CXCL2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), Chemokine (C-X-C Motif) Ligand 2
Isotype
IgG2b
Applications
ELISA Cap
,
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
Purified Recombinant Rat CXCL2 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:C1509 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:C1343.
Western Blotting: To detect Rat CXCL2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1343.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Rat CINC-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat CINC-3 (at 0.1 ng/ml) is 0.4 - 2 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Rat Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 123802) recognizes an epitope on Rat CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.1 This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.2,3,4

Antigen Details

PubMed

References & Citations

1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675
2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010
Elisa Sandwich Protocol
N
General Western Blot Protocol
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Formats Available

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Prod No.
Description
C1433
Products are for research use only. Not for use in diagnostic or therapeutic procedures.