Anti-Rat CXCL2 (Clone 123802)
Anti-Rat CXCL2 (Clone 123802)
Product No.: C1433
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Clone 123802 Target CXCL2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), Chemokine (C-X-C Motif) Ligand 2 Isotype IgG2b Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat CXCL2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828494 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:C1509 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:C1343. Western Blotting: To detect Rat CXCL2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1343. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat CINC-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat CINC-3 (at 0.1 ng/ml) is 0.4 - 2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Rat Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 123802) recognizes an epitope on Rat CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.1 This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.2,3,4 PubMed References & Citations1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675 2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612 3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596 4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010 Technical ProtocolsCertificate of Analysis |
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