Recombinant Human FGF-5

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Pricing & Details

Product. No.F137
Alternate Names
Fibroblast Growth Factor-5, HBGF-5, Smag-82
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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F137-50 µg
50 µg
$219.00
In stock
Max:
Min: 1
Step: 1

Background

Fibroblast growth factor 5, also known as FGF5 is a secreted signaling protein1 that is a member of the fibroblast growth factor gene family.2 FGF5 is widely expressed in embryonic but scarcely in adult tissues.3 FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects.3 FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.4 FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration (ARMD).5

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-5 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Thomas, K. 1987, Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266). The expected ED50 for this effect is typically 2 - 10 ng/ml in the presence of 1 μg/ml of heparin.
Expression Host
E. coli
Protein Accession No.
Q8NF90
Amino Acid Sequence
ekrlapkgqp gpaatdrnpr gsssrqssss amssssasss paaslgsqgs gleqssfqws psgrrtgsly crvgigfhlq iypdgkvngs heanmlsvle ifavsqgivg irgvfsnkfl amskkgklha sakftddckf rerfqensyn tyasaihrte ktgrewyval nkrgkakrgc sprvkpqhis thflprfkqs eqpelsftvt vpekknppsp ikskiplsap rkntnsvkyr lkfrfg
N-terminal Sequence Analysis
Glu23 (without n- terminal Met); Met & Leu26 (with n-terminal Met)
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FGF-5 is Mr 27 kDa.
Formulation
The protein is provided in a 0.2 μm filtered solution of 20 mM MOPS, 50 mM Na2SO4, 0.2 mM EDTA, 0.5 mM DTT, pH 7.0 at a concentration of 0.24 mg/ml.
Storage and Stability
This protein is stable for >12 months when stored at -20°C to -70°C in a manual defrost freezer. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Dry Ice
PubMed
NCBI Gene Bank

References & Citations

1. Martin GR et al. (1997) Cell 78: 1017 2. Thoenen H et al. (1994) Eur J Neurosci. 6: 244 3. Berger W et al. (2008) Oncogene 27: 4180 4. Suzuki S et al. (2003) J Cell Physiol. 197: 272 5. Hjelmeland LM et al. (1997) Curr Eye Res. 16: 396
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
F137
F1091
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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