Recombinant Human FGF-5

Fibroblast growth factor 5, also known as FGF5 is a secreted signaling protein¹ that is a member of the fibroblast growth factor gene family.² FGF5 is widely expressed in embryonic but scarcely in adult tissues.³ FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects.³ FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.⁴ FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration (ARMD).⁵

The biological activity of Human FGF-5 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Thomas, K. 1987, Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266). The expected ED₅₀ for this effect is typically 2 - 10 ng/ml in the presence of 1 μg/ml of heparin.

Q8NF90

ekrlapkgqp gpaatdrnpr gsssrqssss amssssasss paaslgsqgs gleqssfqws psgrrtgsly crvgigfhlq iypdgkvngs heanmlsvle ifavsqgivg irgvfsnkfl amskkgklha sakftddckf rerfqensyn tyasaihrte ktgrewyval nkrgkakrgc sprvkpqhis thflprfkqs eqpelsftvt vpekknppsp ikskiplsap rkntnsvkyr lkfrfg

Glu23 (without n- terminal Met); Met & Leu26 (with n-terminal Met)

Solution

The predicted molecular weight of Recombinant Human FGF-5 is Mr 27 kDa.

27

The protein is provided in a 0.2 μm filtered solution of 20 mM MOPS, 50 mM Na₂SO₄, 0.2 mM EDTA, 0.5 mM DTT, pH 7.0 at a concentration of 0.24 mg/ml.

This protein is stable for >12 months when stored at -20°C to -70°C in a manual defrost freezer. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Dry Ice

1. Martin GR et al. (1997) Cell 78: 1017 2. Thoenen H et al. (1994) Eur J Neurosci. 6: 244 3. Berger W et al. (2008) Oncogene 27: 4180 4. Suzuki S et al. (2003) J Cell Physiol. 197: 272 5. Hjelmeland LM et al. (1997) Curr Eye Res. 16: 396