Recombinant Human FGF-5
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BackgroundFibroblast growth factor 5, also known as FGF5 is a secreted signaling protein1 that is a member of the fibroblast growth factor gene family.2 FGF5 is widely expressed in embryonic but scarcely in adult tissues.3 FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects.3 FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.4 FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration (ARMD).5 Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-5 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Thomas, K. 1987, Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266). The expected ED<sub>50</sub> for this effect is typically 2 - 10 ng/ml in the presence of 1 μg/ml of heparin. Protein Accession No. Q8NF90 Amino Acid Sequence ekrlapkgqp gpaatdrnpr gsssrqssss amssssasss paaslgsqgs gleqssfqws psgrrtgsly crvgigfhlq iypdgkvngs heanmlsvle ifavsqgivg irgvfsnkfl amskkgklha sakftddckf rerfqensyn tyasaihrte ktgrewyval nkrgkakrgc sprvkpqhis thflprfkqs eqpelsftvt vpekknppsp ikskiplsap rkntnsvkyr lkfrfg N-terminal Sequence Analysis Glu23 (without n- terminal Met); Met & Leu26 (with n-terminal Met)
State of Matter Solution Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-5 is Mr 27 kDa. Predicted Molecular Mass 27 Formulation The protein is provided in a 0.2 μm filtered solution of 20 mM MOPS, 50 mM Na2SO4, 0.2 mM EDTA, 0.5 mM DTT, pH 7.0 at a concentration of 0.24 mg/ml. Storage and Stability This protein is stable for >12 months when stored at -20°C to -70°C in a manual defrost freezer. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Dry Ice NCBI Gene Bank References & Citations1. Martin GR et al. (1997) Cell 78: 1017
2. Thoenen H et al. (1994) Eur J Neurosci. 6: 244
3. Berger W et al. (2008) Oncogene 27: 4180
4. Suzuki S et al. (2003) J Cell Physiol. 197: 272
5. Hjelmeland LM et al. (1997) Curr Eye Res. 16: 396 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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