Anti-human FGF-5 – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human FGF-5 (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830138 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human FGF-5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F137. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Fibroblast Growth Factor 5 (FGF-5) recognizes Human FGF-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Fibroblast growth factor 5, also known as FGF5 is a secreted signaling protein1 that is a member of the fibroblast growth factor gene family.2 FGF5 is widely expressed in embryonic but scarcely in adult tissues.3 FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects.3 FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.4 FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration (ARMD).5 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Martin, GR. et al. (1997) Cell 78: 1017 2. Thoenen, H. et al. (1994) Eur J Neurosci. 6: 244 3. Berger, W. et al. (2008) Oncogene 27: 4180 4. Suzuki, S. et al. (2003) J Cell Physiol. 197: 272 5. Hjelmeland, LM. et al. (1997) Curr Eye Res. 16: 396 Technical Protocols |
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