Keratinocyte growth factor (KGF), also known as FGF-7 and HBGF-7, is a member of the fibroblast growth factor (FGF) family. The family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.1 KGF is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.2 When secreted, KGF acts as a paracrine growth factor for nearby epithelial cells. KGF will only signal through the FGF R2-IIIb receptor.3 For signaling to occur, receptor dimerization is required with an octameric or larger heparin or heparin sulfate proteoglycan. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, re-epithelialization of wounds, hair development and early lung organogenesis.4,5 Instillation of KGF into the lungs of animals protects them from oxidant-induced injury to the lung epithelium.
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells.6 The ED50 for this effect is typically 6-60 ng/mL. The specific activity of Recombinant Human FGF-7 is approximately 1.3 x 103 U/μg, which is calibrated against Recombinant Human FGF-7 WHO Standard (NIBSC code: 03/150).
Protein Accession No.
Amino Acid Sequence
cndmtpeqm atnvncsspe rhtrsydyme ggdirvrrlf crtqwylrid krgkvkgtqe mknnynimei rtvavgivai kgvesefyla mnkegklyak kecnedcnfk elilenhynt yasakwthng gemfvalnqk gipvrgkktk keqktahflp mait
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FGF-7 is Mr 19 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Finch, PW. et al. (2006) J. Natl. Cancer Inst. 98:812
2. Aaronson, SA. et al. (1991) Ann. N. Y. Acad. Sci. 638:62
3. Bottaro, DP. et al. (1993) J. Biol. Chem. 268:9180
4. Werner, S. et al. (1992) Proc. Natl. Acad. Sci. (USA) 89:6896
5. Simonet, WS. et al. (1995) Proc. Natl. Acad. Sci. (USA) 92:12461
6. Rubin, JS. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.