Anti-Human FGF-7 (Clone 29522)

Purified Recombinant Human FGF-7 (Accession # P21781)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human FGF-7 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human FGF-7 (at 125 ng/ml) is 1-2 µg/ml.

Mouse Anti-Human Fibroblast Growth Factor 7 (FGF-7) (Clone 29522) recognizes an epitope on Human FGF-7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Fibroblast growth factor 7 (keratinocyte growth factor), also known as FGF7, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. FGF7 plays a significant role in the development of ovarian cancer.¹

1. Ohtake, Y. et al.(2005) Int. J. Oncol. 26: 1209