Recombinant Human Galectin-4

Galectin-4, also known as GAL4, belongs to a family of carbohydrate-binding proteins. It is a transcriptional activator which controls expression of the genes necessary for galactose catabolism. GAL4 is composed of two carbohydrate recognition domains within the same peptide chain. Both domains bind lactose with a similar affinity as other galectins but their respective preferences for other disaccharides, and larger saccharides, are distinctly different (1). During development and in adult normal tissues GAL4 is expressed only in the alimentary tract from the tongue to the large intestine (2). It is often found in relatively insoluble complexes, as a component of either adherens junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures (3). GAL4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues. However, strong expression of GAL4 can be induced in cancers from other tissues including breast and liver (4). In cultured cells, intracellular GAL4 may promote resistance to nutrient starvation, whereas, as an extracellular protein, it can mediate cell adhesion. Because of its distinct induction in breast and other cancers, it may be a valuable diagnostic marker and target for the development of inhibitory carbohydrate-based drugs.

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Ala2

Lyophilized

The predicted molecular weight of Recombinant Human Galectin-4 is Mr 36 kDa.

36

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS)and Betamercaptoethanol pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Yang, RY. et al. (2008) Expert Rev. Mol. Med.10:17
2. Huflejt, ME. et al. (2004) Glycoconj. J. 20:247
3. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679
4. Elola, MT. et al. (2007) Cell. Mol. Life Sci. 64:1679