Recombinant Human Galectin-7

Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Galectin-7 (Gal-7) is highly expressed in the epidermis and contributes to different events associated with the differentiation and development of pluristratified epithelia (1). Gal-7 is also associated with epithelial cell migration, which plays a crucial role in the re-epithelialization process of corneal or epidermal wounds. In addition, recent evidence indicates that Gal-7, designated as the product of the p53-induced gene 1 (PIG1), is a regulator of apoptosis through JNK activation and mitochondrial cytochrome c release (2). Defects in apoptosis constitute one of the major hallmarks of human cancers and Gal-7 can act as either a positive or a negative regulatory factor in tumor development, depending on the histological type of the tumor. Specifically, this protein has been shown to increase susceptibility of keratinocytes to UVB induced apoptosis, an essential process in the maintenance of epidermal homeostasis (3).

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Ser2

Lyophilized

The predicted molecular weight of Recombinant Human Galectin-7 is Mr 15 kDa.

15

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS)and Betamercaptoethanol with Trehalose. pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Saussez, S. et al. (2006) Cell. Mol. Life Sci. 63:686
2. Bernerd, F. et al. (1999) Proc. Nat. Acad. Sci. (USA) 96:11329
3. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487