Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes.1,2 It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.2 CXCL6 possesses antibacterial activity against Gram-positive and Gram-negative pathogenic bacteria of relevance to mucosal infections. CXCL6 causes membrane disruption in a dose-dependent manner. This is reduced in the presence of NaCl, which inhibits the binding of the peptide to the bacterial surface.3 CXCL6, in addition to its chemotactic and angiogenic properties, is likely to contribute to direct antibacterial activity during localized infection and thus plays a role in innate immunity.
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human GCP-2 was determined by its ability to induce myeloperoxidase release from cytochalasin-B treated human neutrophils (Schröder, J.M., et al. 1987, J. Immunol. 139:3474) or to induce chemotaxis of mouse BaF/3 cells transfected with hCXCR2. The expected ED50 for these effects are typically 0.5 - 1.5 μg/ml and 3 - 15 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
vsavltelrc tclrvtlrvn pktigklqvf pagpqcskve vvaslkngkq vcldpeapfl kkviqkilds gnkkn
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human GCP-2 is Mr 8 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Van Damme, J. et al. (1993)Biochemistry 32: 10170
2. Van Damme, J. et al. (1997)Biochemistry 36: 2716
3. Linge, HM. et al. (2008)Antimicrobial Agents And Chemotherapy 52: 2599
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.