Anti-Human CXCL6
Anti-Human CXCL6
Product No.: C1398
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Clone 60910 Target CXCL6 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names GCP-2, SCYB6, CKA-3 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CXCL6 (Accession # P80162) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828455 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CXCL6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL6 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G130. ELISA Capture: Suitable for use at concentration of2-8 µg/mL. Use in combination with: Detection Reagent: Human CXCL6/GCP‑2 Biotinylated Antibody. Standard: Recombinant Human CXCL6/GCP-2 Protein. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human CXCL6 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL6 (at 0.08 ng/ml) is 8-30 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human CXCL6 (Clone 60910) recognizes an epitope on Human CXCL6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In sandwich ELISAs, less than 0.04% cross-reactivity was observed with rhNAP-2, rhGROα, rhGROβ, rhBLC, rhENA-78, rmCXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1α and rhSDF-1β. Background Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes.1,2 It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.2 CXCL6 possesses antibacterial activity against Gram-positive and Gram-negative pathogenic bacteria of relevance to mucosal infections. CXCL6 causes membrane disruption in a dose-dependent manner. This is reduced in the presence of NaCl, which inhibits the binding of the peptide to the bacterial surface.3 CXCL6, in addition to its chemotactic and angiogenic properties, is likely to contribute to direct antibacterial activity during localized infection and thus plays a role in innate immunity. Antigen DetailsPubMed References & Citations1. Van Damme, J. et al. (1993)Biochemistry 32: 10170 2. Van Damme, J. et al. (1997)Biochemistry 36: 2716 3. Linge, HM. et al. (2008)Antimicrobial Agents And Chemotherapy 52: 2599 Technical Protocols N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
