Purified Recombinant Human CXCL6 (Accession # P80162)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CXCL6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CXCL6 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G130.
ELISA Capture: Suitable for use at concentration of2-8 µg/mL. Use in combination with: Detection Reagent: Human CXCL6/GCP‑2 Biotinylated Antibody. Standard: Recombinant Human CXCL6/GCP-2 Protein.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CXCL6 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL6 (at 0.08 ng/ml) is 8-30 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CXCL6 (Clone 60910) recognizes an epitope on Human CXCL6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In sandwich ELISAs, less than 0.04% cross-reactivity was observed with rhNAP-2, rhGROα, rhGROβ, rhBLC, rhENA-78, rmCXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1α and rhSDF-1β.
Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes.1,2 It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.2 CXCL6 possesses antibacterial activity against Gram-positive and Gram-negative pathogenic bacteria of relevance to mucosal infections. CXCL6 causes membrane disruption in a dose-dependent manner. This is reduced in the presence of NaCl, which inhibits the binding of the peptide to the bacterial surface.3 CXCL6, in addition to its chemotactic and angiogenic properties, is likely to contribute to direct antibacterial activity during localized infection and thus plays a role in innate immunity.
References & Citations
1. Van Damme, J. et al. (1993)Biochemistry 32: 10170
2. Van Damme, J. et al. (1997)Biochemistry 36: 2716
3. Linge, HM. et al. (2008)Antimicrobial Agents And Chemotherapy 52: 2599
Products are for research use only. Not for use in diagnostic or therapeutic procedures.