Recombinant Human GFRα-1

GDNF family receptor alpha 1, also known as GFRA1 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRalpha1 functions as a ligand-induced cell adhesion molecule (LICAM) to establish precise synaptic contacts and induce presynaptic differentiation.¹

The biological activity of Human GFRα-1 was determined by its ability to bind recombinant human GDNF. Immobilized recombinant human GFRα-1/Fc at 2 μg/ml (100 μl/well) can bind recombinant human GDNF with a linear range at 0.78 - 5 ng/ml in a functional ELISA assay.

Fc Fusion Protein

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Ala19

Lyophilized

The predicted molecular weight of Recombinant Human GFRα-1 is Mr 73.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 88-95 kDa.

73.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Ibáñez, CF. et al. (2007)Nat Neurosci. 10 : 293