Recombinant Human GFRα-1

Recombinant Human GFRα-1

Product No.: G653

[product_table name="All Top" skus="G653"]

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Alternate Names
Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 1, GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, MGC23045, RET1L, RETL1, TRNR1
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Human
Applications
ELISA Cap

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Product Size
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Background

GDNF family receptor alpha 1, also known as GFRA1 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRalpha1 functions as a ligand-induced cell adhesion molecule (LICAM) to establish precise synaptic contacts and induce presynaptic differentiation.1

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GFRα-1 was determined by its ability to bind recombinant human GDNF. Immobilized recombinant human GFRα-1/Fc at 2 μg/ml (100 μl/well) can bind recombinant human GDNF with a linear range at 0.78 - 5 ng/ml in a functional ELISA assay.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
ae vsggdrldcv kasdqclkeq scstkyrtlr qcvagketnf slasgleakd ecrsamealk qkslyncrck rgmkkekncl riywsmyqsl qgndlledsp yepvnsrlsd ifrvvpfisv ehipkgnncl daakacnldd ickkyrsayi tpcttsvsnd vcnrrkchka lrqffdkvpa khsygmlfcs crdiacterr rqtivpvcsy eerekpncln lqdscktnyi crsrladfft ncqpesrsvs sclkenyadc llaysgligt vmtpnyidss slsvapwcdc snsgndleec lkflnffkdn tclknaiqaf gngsdvtvwq pafpvqttta ttttalrvkn kplgpagsen eipthvlppc anlqaqklks nvsgnthlci sngnyekegl gasshittki egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgkhhhh hh
N-terminal Sequence Analysis
Ala19
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human GFRα-1 is Mr 73.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 88-95 kDa.
Predicted Molecular Mass
73.3
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Ibáñez, CF. et al. (2007)Nat Neurosci. 10 : 293
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.