Recombinant Human Growth Hormone Receptor

Growth hormone receptor, also known as GHR is a 54 kDa transmembrane receptor and a member of the class I cytokine receptor family. Biologically active growth hormone binds to GHR, which dimerizes to activate an intracellular signal transduction pathway leading to synthesis and secretion of IGF1. In plasma, IGF1 binds to the soluble IGF1 receptor (IGF1R). At target cells, this complex activates signal-transduction via the JAK/STAT pathway, which results in the mitogenic and anabolic responses that lead to growth (1). GHR has also been shown to interact with SGTA, PTPN11 and CISH (2-4). Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism) (5).

The biological activity of Human GHR was determined by its ability to inhibit GH-induced proliferation of Nb211 rat lymphoma cells. Gout, P.W. et al., (1980) Cancer Res. 40:2433. The expected ED₅₀= 0.4 - 2.0 ng/ml in the presence of 0.2 ng/ml of rhGH.

Fc Fusion Protein

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Ala27

Lyophilized

The predicted molecular weight of Recombinant Human GHR is Mr 54 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-90 kDa.

54

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Goffin, V. et al. (1996) Endocrine Rev. 17:385
2. Schantl, J. et al. (2003) Biochem. J. 373:85
3. Stofega, MR. et al. (2000) Mol. Endocrinol. 14:1338
4. Ram, PA. et al. (1999) J. Biol. Chem. 274:35553
5. Laron, Z. et al. (2004) J. Clin. Endocrinol. Metab. 89:1031