Recombinant Rat Growth Hormone Receptor

Recombinant Rat Growth Hormone Receptor

Product No.: G644

[product_table name="All Top" skus="G644"]

- -
- -
Alternate Names
Growth Hormone Receptor, GHR/BP, MGC124963, MGC156665
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Rat

- -
- -
Product Size
- -
- -

Background

Growth hormone receptor, also known as GHR is a 55 kDa transmembrane receptor and a member of the class I cytokine receptor family. Biologically active growth hormone binds to GHR, which dimerizes to activate an intracellular signal transduction pathway leading to synthesis and secretion of IGF1. In plasma, IGF1 binds to the soluble IGF1 receptor (IGF1R). At target cells, this complex activates signal-transduction via the JAK/STAT pathway, which results in the mitogenic and anabolic responses that lead to growth (1). GHR has also been shown to interact with SGTA, PTPN11 and CISH (2-4). Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism) (5).

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
fp gsgatpatlg kaspvlqrin pslresssgk prftkcrspe letfscywte gddhnlkvpg siqlyyarri ahewtpewke cpdyvsagan scyfnssyts iwipyciklt tngdlldekc ftvdeivqpd ppiglnwtll nislpgirgd iqvswqppps advlkgwiil eyeiqykevn etkwktmspi wstsvplysl rldkehevrv rsrqrsfeky sefsevlrvt fpqmdtlaac eedfrriegr mdpkscdkth tcppcpapel lggpsvflfp pkpkdtlmis rtpevtcvvv dvshedpevk fnwyvdgvev hnaktkpree qynstyrvvs vltvlhqdwl ngkeykckvs nkalpapiek tiskakgqpr epqvytlpps rdeltknqvs ltclvkgfyp sdiavewesn gqpennyktt ppvldsdgsf flyskltvdk srwqqgnvfs csvmhealhn hytqkslsls pgk
N-terminal Sequence Analysis
Phe19
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat GHR is Mr 55.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 65-80 kDa.
Predicted Molecular Mass
55.1
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Goffin, V. et al. (1996) Endocrine Rev. 17:385
2. Schantl, J. et al. (2003) Biochem. J. ,/i> 373: 85
3. Stofega, MR. et al. (2000) Mol. Endocrinol. 14:1338
4. Ram, PA. et al. (1999) J. Biol. Chem. 274:35553
5. Laron, Z. et al. (2004) J. Clin. Endocrinol. Metab. 89:1031
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.