Recombinant Human Growth Hormone Receptor

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Pricing & Details

Product. No.G643
Alternate Names
Growth Hormone Receptor, GHBP
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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G643-50 µg
50 µg
$379.00
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Min: 1
Step: 1

Background

Growth hormone receptor, also known as GHR is a 54 kDa transmembrane receptor and a member of the class I cytokine receptor family. Biologically active growth hormone binds to GHR, which dimerizes to activate an intracellular signal transduction pathway leading to synthesis and secretion of IGF1. In plasma, IGF1 binds to the soluble IGF1 receptor (IGF1R). At target cells, this complex activates signal-transduction via the JAK/STAT pathway, which results in the mitogenic and anabolic responses that lead to growth (1). GHR has also been shown to interact with SGTA, PTPN11 and CISH (2-4). Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism) (5).

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GHR was determined by its ability to inhibit GH-induced proliferation of Nb211 rat lymphoma cells. Gout, P.W. et al., (1980) Cancer Res. 40:2433. The expected ED50= 0.4 - 2.0 ng/ml in the presence of 0.2 ng/ml of rhGH.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
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N-terminal Sequence Analysis
Ala27
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human GHR is Mr 54 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-90 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Goffin, V. et al. (1996) Endocrine Rev. 17:385
2. Schantl, J. et al. (2003) Biochem. J. 373:85
3. Stofega, MR. et al. (2000) Mol. Endocrinol. 14:1338
4. Ram, PA. et al. (1999) J. Biol. Chem. 274:35553
5. Laron, Z. et al. (2004) J. Clin. Endocrinol. Metab. 89:1031
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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