Recombinant Human Heregulin-β1 (HRG1-β EGF Domain)

Neuregulin/Heregulin is a family of related growth factors that are differentially spliced variants of four genes: NRG1, NRG2, NRG3 and NRG4. The longest forms of NRG contains several different modular domains and tissue-specific splicing results in many NRG isoforms containing different sets of these domains. Alternative splicing produces two types of EGF domain, designated a and b. HRG-Beta1 contains an Ig domain and an EGF domain necessary for direct binding to receptor tyrosine kinases erbB-3 and erbB-4. HRG-Beta1 binding causes erbB-3 and erbB-4 to dimerize with erbB-2 (Her2/neu) and thereby regulates the phosphorylation of erbB-2 tyrosines. HRG-Beta1 stimulates proliferation and motility of breast cancer cells and plays a role in wound healing by stimulating epidermal migration and differentiation of epidermal cells and by stimulating the expression of integrins in the epidermis.

The biological activity of Human HRG-Beta1 was determined by its ability to stimulate the proliferation of human MCF-7 cells grown under serum-free conditions.¹ The expected ED₅₀ for this effect is typically 0.5 - 2 ng/ml.

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Thr176

Lyophilized

The predicted molecular weight of Recombinant Human HRG1-Beta1 is Mr 8 kDa. Additionally, the actual molecular weight as observed by migration on SDS-PAGE is Mr 8 kDa (reducing conditons).

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Karey, KP. et al. (1988) Cancer Research 48:4083.