Recombinant Human NRG1-β1

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Pricing & Details

Product. No.N188
Alternate Names
HRG1-β1, Heregulin 1-β1 Extracellular Domain, HRG, Breast Cancer Cell Differentiation Factor p45, Acetylcholine Receptor Inducing Activity [ARIA], Glial Factor, Neu Differentiation Factor
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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N188-50 µg
50 µg
$349.00
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Data

Background

Neuregulin/Heregulin is a family of related growth factors that are differentially spliced variants of four genes: NRG1, NRG2, NRG3 and NRG4. The longest forms of NRG contains several different modular domains and tissue-specific splicing results in many NRG isoforms containing different sets of these domains. Alternative splicing produces two types of EGF domain, designated a and b. HRG-Beta1 contains an Ig domain and an EGF domain necessary for direct binding to receptor tyrosine kinases erbB-3 and erbB-4. HRG-Beta1 binding causes erbB-3 and erbB-4 to dimerize with erbB-2 (Her2/neu) and thereby regulates the phosphorylation of erbB-2 tyrosines. HRG-Beta1 stimulates proliferation and motility of breast cancer cells and plays a role in wound healing by stimulating epidermal migration and differentiation of epidermal cells and by stimulating the expression of integrins in the epidermis.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human NRG1-β1 was determined a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells. The expected ED50 for this effect is typically 2.5 - 12.5 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
serkegrgk gkgkkkergs gkkpesaags qspalppqlk emksqesaag sklvlrcets seysslrfkw fkngnelnrk nkpqnikiqk kpgkselrin kasladsgey mckvisklgn dsasanitiv esneiitgmp astegayvss espirisvst egantsssts tsttgtshlv kcaekektfc vnggecfmvk dlsnpsrylc kcpneftgdr cqnyvmasfy khlgiefmea eelyqk
N-terminal Sequence Analysis
Ser2
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human NRG1-β1 is Mr 26.9 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Adlkofer, K. and C. Lai (2000) Glia 29:104
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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