Recombinant Human IgG

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Pricing & Details

Product. No.I-539
Alternate Names
Immunoglobulin G
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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I-539-100 µg
100 µg
$329.00
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Background

Immunoglobulin G (IgG) is a monomeric immunoglobulin synthesized and secreted by plasma B cells. It is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids (1). IgG antibodies are predominately involved in the secondary antibody response (the main antibody involved in primary response is IgM) (2). Pro-inflammatory cytokines particularly IL-4 and IL-2 have a crucial role in activation of the IgG antibody response. IgG is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero (3). In addition, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
This protein is meant for use as a control.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
iegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk
N-terminal Sequence Analysis
Ile
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IgG is Mr 26.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-35 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Junqueira, LC. and Carneiro, J. (2003) Basic Histology. Tenth edition, McGraw Hill Companies
2. Meulenbroek, AJ. et al. (1996) Human IgG Subclasses: Useful diagnostic markers for immunocompetence (Sanquin)
3. Pitcher-Wilmott, RW. et al. (1980) Clin. Exp. Immunol. 41:303
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
I-539
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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