Recombinant Human IL-17E

Interleukin-17E (IL-17E), also known as IL-25, belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. It is secreted by type 2 helper T cells and mast cells. IL-17E stimulates secretion of IL-8 and induces activation of the transcription factor NK-κB in cells that express the IL-17BR receptor.¹ IL-17E induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues. IL-17E is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth.² In addition, studies have shown that enforced expression of IL-17E in the lung significantly enhances antigen-induced Th2 cytokine production and eosinophil recruitment into the airways. This suggests an important role of IL-17E in enhancing allergic airway inflammation by a CD4+ T-cell-dependent mechanism.³ IL-17E is also an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.⁴

The biological activity of Human IL-17E was determined by its ability to bind with rhIL-17BR/Fc in a functional ELISA.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human IL-17E is Mr 17 kDa.

17

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Gurney, AL. et al. (2001) J. Biol. Chem 276:1660
2. Gurney, AL. et al. (2001) J. Immunol. 167:6559
3. Hamid, Q. et al. (2006) J. Allergy Clin. Immunol. 117:590
4. Owyang, AM. et al. (2006) J. Exp. Med. 203:843