Recombinant Human IL-20

Interleukin-20 (IL-20) is a pleiotropic inflammatory protein belonging to the IL-10 family of cytokines. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 is capable of stimulating colony formation by CD34+ multipotential progenitors but not by other progenitor cells. ¹ It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.² Human IL-20 is active on mouse cells.

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 Rα and human IL-20 Rβ. The ED₅₀ for this effect is 0.05-0.3 ng/mL.

AAF36679

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Met

Lyophilized

The predicted molecular weight of Recombinant Human IL-20 is Mr 17.6 kDa.

17.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein. pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Kupper, TS. et al. (2006) Anti-Inflamm. & Anti-Allergy Agents Med. Chem. 5:243
2. Dam, TM. et al. (2009) Br. J. Dermatol. 160:284