Recombinant Human IL-8, CXC Chemokine (77 a.a.)

Recombinant Human IL-8, CXC Chemokine (77 a.a.)

Product No.: I-191

[product_table name="All Top" skus="I-191"]

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Prod. No.I-191
Expression Host
E. coli Cells

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Background

Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. IL-8 is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Gene of IL-8 is believed to play a role in the pathogenesis of bronchiolitis. While neutrophil granulocytes are the primary target cells of IL-8 there is a relative wide range of cells (endothelial cells, macrophages, mast cells, keratinocytes) responding to this chemokine, too. When first encountering an antigen, the primary cells to encounter it are the macrophages that phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor. Interleukin-8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in psoriasis.1

Protein Details

Purity
>95% by SDS-PAGE and HPLC
Endotoxin Level
<1.0 EU/µg
Biological Activity
The biological activity of Human Interleukin-8 (77 a.a.) is determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 25.0-150.0 ng/ml.
Protein Accession No.
Amino Acid Sequence
AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA

References & Citations

1. Haraldsen G et al. (1998) J Exp Med. 188: 1751
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.