Interleukin 8 (IL-8), also known as CXCL8, is a member of the CXC chemokine family. It is secreted by a variety of cell types including monocytes, macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL-1, TNF, LPS) (1). It is also synthesized by endothelial cells, which store IL-8 in vesicles. IL-8 functions as a chemoattractant for neutrophils, basophils, and T cells, but not monocytes (2). It also plays a vital role in tumorigenesis, progression and metastasis, and accordingly the expression levels of IL-8 correlate with disease progression in several human carcinomas (3). IL-8 has the N-terminal tripeptide ELR motif, which is known to mediate IL-8 receptor binding and subsequent signal transduction, such as the TRAF6-NF-κB pathway. It can also signal through the CXCR1 and CXCR2 receptors. IL-8 is believed to play a role in the pathogenesis of cystic fibrosis (4).
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human IL-8 was determined by its induction of myeloperoxidase release from human neutrophils (Schröeder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of hCXCR-2 transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 0.15 - 0.3 μg/ml, or 0.5 - 2.5 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
sak elrcqcikty skpfhpkfik elrviesgph canteiivkl sdgrelcldp kenwvqrvve kflkraens
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-8 is Mr 8 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in deionized water.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Baggiolini, M. et al. (1992) FEBS Lett. 307:97
2. Köhidai, L. et al. (1998) Cytokine 10:481
3. Yuan, A. et al. (2006) Front. Biosci. 10:853
4. Tabary, O. et al. (1998) Amer. J. Pathol. 153:921
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.