Recombinant Human NT-4

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Pricing & Details

Product. No.N118
Alternate Names
Neurotrophin-4, Neurotrophin 5, NT5, NTF5, Neutrophic Factor 5, Neutrophic Factor 4
Expression Host
Sf 21 Insect Cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
N118-5 µg
5 µg
$239.00
In stock
Max:
Min: 1
Step: 1
N118-25 µg
25 µg
$699.00
In stock
Max:
Min: 1
Step: 1

Background

Neurotrophin 4 (NT-4), also known as NT-5, is a member of the NGF family and is related to β-NGF, BDNF and NT-3.1 It promotes the development and survival of selected peripheral and CNS neurons. Several aspects distinguish NT-4 from other members of the neurotrophin family. It is the most divergent member and, in contrast to the other neurotrophins, its expression is ubiquitous and appears to be less influenced by environmental signals. NT-4 levels are highest in the prostate, with lower levels in thymus, placenta and skeletal muscle. It signals predominantly through the TrkB receptor tyrosine kinase. Knock-out mice lacking expression of NT-4 show long-term memory defects.3 In addition, NT-4 promotes the proliferation of keratinocytes and accelerates hair follicle regression.4 NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration.5

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human NT-4 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 5 - 15 ng/ml.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
gvsetapasr rgelavcdav sgwvtdrrta vdlrgrevev lgevpaaggs plrqyffetr ckadnaeegg pgaggggcrg vdrrhwvsec kakqsyvral tadaqgrvgw rwiridtacv ctllsrtgra
N-terminal Sequence Analysis
Gly81
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human NT-4 is Mr 14 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Persson, H. et al. (1993) Development 117:1345
2. Ibanez, CF. et al. (1996) Neurochem. Sci. 21:787
3. Xie, CW. et al. (2000) Proc. Natl. Acad. Sci. (USA) 97:8116
4. Botchkarev, VA. et al. (1999) Lab. Invest. 79:557
5. Laurenzi, MA. et al. (1988) J. Leukoc. Biol. 64:228
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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