Anti-Human NT-4 (Clone 36526)
Anti-Human NT-4 (Clone 36526)
Product No.: N201
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Clone 36526 Target NT-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Neurotrophic Factors-4, Neurotrophin 5, NT5, NTF5, Neutrophic Factor 5, Neutrophic Factor 4 Isotype IgG1 Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human NT-4 (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831434 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:N129 at a concentration of approximately 100 µg/ml. A suggested standard for this assay is PN:N118. Western Blotting: To detect Human NT-4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human NT-4 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:N118. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Neurotrophic Factors 4 (NT-4) (Clone 36526) recognizes an epitope on Human NT-4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with rhNT-3 and rhBDNF. Background Neurotrophin 4 (NT-4) is a novel neurotrophic factor1, the neurotrophins, that control survival and differentiation of vertebrate neurons. Several aspects distinguish NT-4 from other members of the neurotrophin family. It is the most divergent member and, in contrast to the other neurotrophins, its expression is ubiquitous and appears to be less influenced by environmental signals. NT-4 seems to have the unique requirement of binding to the low-affinity neurotrophin receptor for efficient signalling and retrograde transport in neurons.2 NT-4 controls survival and differentiation of mammalian neurons. It signals predominantly through the TrkB receptor tyrosine kinase. Both NT-4/5 and brain-derived neurotrophic factor may be physiological survival factors for facial motor neurons and may serve as therapeutic agents for motor neuron disease.3 Antigen DetailsPubMed References & Citations1. Persson, H. et al. (1993) Development 117: 1345 2. Ibanez, CF. et al. (1996) Neurochemical Scie. 21: 787 3. Rosenthal, A. et al. (1994) Proc Natl Acad Sci USA 91: 3304 Technical Protocols  |
Related Products
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Prod No. | Description |
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N201 | |
N118 | |
N129 |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
