Recombinant Human PDGF-BB

Recombinant Human PDGF-BB is widely used in research due to its potent ability to stimulate cell proliferation, migration, and tissue regeneration, making it valuable for studies in wound healing, tissue engineering, and regenerative medicine.

Key scientific reasons to use recombinant human PDGF-BB in research applications include:

• Potent Mitogenic and Chemotactic Effects: PDGF-BB is a strong mitogen (stimulates cell division) and chemoattractant for cells of mesenchymal origin, such as fibroblasts, smooth muscle cells, mesenchymal stem cells (MSCs), osteogenic cells, and tenocytes. This makes it essential for studies involving cell proliferation, migration, and differentiation.

• Promotion of Angiogenesis: PDGF-BB upregulates angiogenesis (formation of new blood vessels) by stimulating vascular endothelial growth factor (VEGF) and other pathways, which is critical for tissue repair and regeneration studies.

• Tissue Regeneration and Wound Healing: Recombinant human PDGF-BB is the only growth factor approved by the FDA for tissue regeneration and wound healing, with proven efficacy in promoting healing of chronic wounds, bone defects, and soft tissue injuries. It enhances extracellular matrix production (collagen, elastin, fibronectin) and revascularization, supporting tissue remodeling and repair.

• Anti-Senescence and Cytoprotective Effects: Recent studies show that PDGF-BB can mitigate cellular senescence, reduce oxidative stress, and promote cell cycle progression, making it a promising candidate for research on aging, degenerative diseases, and cellular homeostasis.

• Safety Profile: Extensive preclinical and clinical data demonstrate that recombinant human PDGF-BB is safe, with no evidence of toxicity, carcinogenicity, or tumor promotion when used as indicated in research and clinical settings.

• Versatility in Experimental Applications: Recombinant PDGF-BB is used in a variety of research contexts, including:

  • Cell culture and differentiation studies (e.g., MSC development)
  • Functional assays for cell proliferation and migration
  • Angiogenesis assays
  • Tissue engineering and regenerative medicine models

• Consistency and Reproducibility: Recombinant production ensures batch-to-batch consistency, purity, and defined activity, which are critical for reproducible experimental results.

In summary, recombinant human PDGF-BB is a scientifically validated, safe, and versatile growth factor that supports a wide range of research applications focused on cell biology, tissue regeneration, and disease modeling.

You can use recombinant human PDGF-BB as a standard for quantification or calibration in ELISA assays, provided that the recombinant protein is of high purity, its concentration is accurately determined, and it is compatible with the antibodies and detection system used in your assay.

Key considerations:

• Equivalence to Natural Protein: Many commercial ELISA kits use recombinant human PDGF-BB as the standard, and studies have shown that recombinant and natural PDGF-BB yield parallel standard curves, indicating comparable immunoreactivity and suitability for quantification.
• Calibration and Traceability: For highest accuracy, standards should ideally be calibrated against a recognized reference such as the WHO International Standard (NIBSC code: 94/728). If your recombinant PDGF-BB is traceable to such a standard, this enhances the reliability of quantification.
• Purity and Quantification: Ensure your recombinant PDGF-BB is highly purified and its concentration is determined by a reliable method (e.g., absorbance at 280 nm, amino acid analysis, or quantitative ELISA).
• Buffer and Matrix Effects: Prepare your standard in the same buffer or matrix as your samples to minimize matrix effects and ensure accurate calibration.
• Validation: It is good practice to validate that your recombinant standard produces a standard curve that is linear and parallel to the curve generated by the kit’s original standard, especially if you are substituting it in a commercial kit.

Summary Table: Use of Recombinant PDGF-BB as ELISA Standard

In practice:
If your recombinant PDGF-BB meets these criteria, it is suitable for use as a standard in ELISA quantification or calibration. Always document your standard’s source, lot, and calibration method for reproducibility and regulatory compliance.

Recombinant Human PDGF-BB has been validated in published research for a wide range of applications, primarily in the fields of tissue repair, regenerative medicine, cell biology, and disease modeling.

Key validated applications include:

• Tissue Repair and Regeneration: Extensively used to promote wound healing, bone regeneration, and connective tissue repair. It is FDA-approved for clinical use in healing diabetic skin wounds, intra-oral tissue regeneration, and orthopedic bone/tissue regeneration. Studies confirm its efficacy in stimulating fibroblast and smooth muscle cell proliferation, granulation tissue formation, and extracellular matrix remodeling.

• Cell Culture and Functional Assays: Used as a growth factor in cell culture to stimulate proliferation, migration, and differentiation of various cell types, including mesenchymal stem cells (MSCs), fibroblasts, smooth muscle cells, and neural cells. It is also applied in functional assays to study signaling pathways such as PI3K/Akt and MAPK.

• Angiogenesis Studies: Validated for promoting angiogenesis by recruiting pericytes and stabilizing blood vessels, making it a standard tool in vascular biology and tissue engineering research.

• Anti-Senescence and Degeneration Models: Demonstrated to alleviate cellular senescence and oxidative stress in primary human intervertebral disc cells and dermal fibroblasts, and to restore biomechanical function in animal models of disc degeneration. It also mitigates senescence in MSCs and other cell types.

• Neuroscience and Neuroregeneration: Investigated for its neuroprotective and regenerative effects, including increasing dopamine transporter binding and stimulating progenitor cell proliferation in models of Parkinson’s disease.

• Immunomodulation and Inflammation: Shown to modulate immune cell activity, including chemotaxis of neutrophils and mononuclear cells, stimulation of cytokine secretion, and regulation of T cell responses.

• Bioassays and Protein Detection: Used as a standard in bioassays for protein detection and quantification, including ELISA and ultrasensitive protein detection platforms.

• Disease Modeling and Mechanistic Studies: Applied in studies of vascular homeostasis, cancer cell biology, and chronic disease models to elucidate PDGF-BB’s role in cell signaling, proliferation, and tissue remodeling.

Summary Table: Validated Applications of Recombinant Human PDGF-BB

These applications are supported by both preclinical and clinical studies, as well as widespread use in cell biology and tissue engineering research.

To reconstitute and prepare Recombinant Human PDGF-BB protein for cell culture experiments, follow these best-practice steps:

1. Centrifuge the vial briefly before opening to ensure all lyophilized material is at the bottom.

2. Reconstitution:

   • Add sterile water or sterile 100 mM acetic acid containing 0.1% BSA to the vial to achieve a concentration of 0.1–1.0 mg/mL.
   • If using water, a typical starting concentration is 0.1 mg/mL.
   • For some protocols, 4 mM HCl or 100 mM acetic acid with 0.1% BSA may be used to maximize solubility and stability.
   • Gently pipette the solution down the sides of the vial to dissolve the protein. Do not vortex.

3. Aliquoting and Storage:

   • Once fully dissolved, aliquot the solution into small working volumes to avoid repeated freeze-thaw cycles.
   • Store aliquots at ≤ –20°C for long-term storage or at 4°C for short-term use (up to 1 month).
   • For extended storage, some protocols recommend –80°C.

4. Dilution for Cell Culture:

   • For working concentrations, dilute the reconstituted stock in cell culture medium or aqueous buffer containing 0.1% BSA or HSA to prevent adsorption and loss of activity.
   • Avoid repeated freeze-thaw cycles, as this can reduce protein activity.

5. General Handling Tips:

   • The protein may appear as a thin film; ensure complete dissolution by gentle mixing.
   • Use sterile technique throughout to prevent contamination.

Summary Table: Key Steps and Conditions

Note: Always consult the specific product datasheet for any unique recommendations regarding buffer composition or concentration, as minor differences may exist between preparations. If using for sensitive cell types, confirm that all reagents (including BSA) are cell culture grade and endotoxin-free.