Recombinant Human SCF

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Pricing & Details

Product. No.S109
Alternate Names
Stem Cell Factor, KITLG, Steel Factor (SF), DKFZp686F2250, KL-1, Kitl, Mast Cell Growth Factor (MGF)
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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S109-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
S109-50 µg
50 µg
$639.00
In stock
Max:
Min: 1
Step: 1
S109-200 µg
200 µg
$929.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Stem cell factor (SCF), also known as KIT ligand, MGF and steel factor, is a type I transmembrane glycoprotein and hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis (1). It is produced by the Sertoli cells and is involved in the local regulation of spermatogenesis and liver regeneration (2). Non-covalent forms of SCF interact with the receptor tyrosone kinase c-Kit (CD117) to trigger receptor dimerization and signaling (3). The activation of c-Kit subsequently activates multiple signaling cascades, including the RAS/ERK, PI3-Kinase, Src kinase and JAK/STAT pathways (4). SCF, in conjunction with IL-31, may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung (5). It also mediates cell to cell adhesion, again by acting synergistically with other cytokines. A soluble form of SCF is produced by proteolytic processing of the molecule in the extracellular domain (6). Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human SCF was measured in a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 2.5 - 10 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
megicr nrvtnnvkdv tklvanlpkd ymitlkyvpg mdvlpshcwi semvvqlsds ltdlldkfsn iseglsnysi idklvnivdd lvecvkenss kdlkksfksp eprlftpeef frifnrsida fkdfvvaset sdcvvsstls pekdsrvsvt kpfmlppva
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human SCF is Mr 18.6 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.