Recombinant Mouse SCF

Recombinant Mouse SCF

Product No.: S112

[product_table name="All Top" skus="S112"]

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Prod. No.S112
Expression Host
E. coli Cells

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Background

Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse SCF was determined by a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED<sub>50</sub> for this effect is typically 2.5-10 ng/ml. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin.
Protein Accession No.
Q78ED8
Amino Acid Sequence
mkeicg npvtdnvkdi tklvanlpnd ymitlnyvag mdvlpshcwl rdmviqlsls lttlldkfsn iseglsnysi idklgkivdd lvlcmeenap knikespkrp etrsftpeef fsifnrsida fkdfmvasdt sdcvlsstlg pekdsrvsvt kpfmlppva
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse SCF is Mr 18.6 kDa.
Predicted Molecular Mass
18.6
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS)and sodium chloride (NaCl) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient

References & Citations

1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.