Recombinant Mouse SCF

Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor¹ is a paracrine regulator of germ cell development.² SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.³ SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis⁴ and liver regeneration.⁵ SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.⁶

The biological activity of Mouse SCF was determined by a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED₅₀ for this effect is typically 2.5-10 ng/ml. The cell number is assessed in a fluorometric assay using the redox sensitive dye, Resazurin.

Q78ED8

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Met

Lyophilized

The predicted molecular weight of Recombinant Mouse SCF is Mr 18.6 kDa.

18.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS)and sodium chloride (NaCl) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390