Recombinant Human SDF-1β

Recombinant Human SDF-1β

Product No.: S110

[product_table name="All Top" skus="S110"]

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Alternate Names
Stromal Cell-Derived Factor-1β, Thymic Lymphoma Cell Stimulating Factor-Beta (TLSF-β), CXCL12, Pre-B Cell Growth-Stimulating Factor (PBSF)
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human/Feline

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Product Size
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Background

SDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.1 SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.2

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human SDF-1β was determined by its ability to chemoattract mouse BaF/3 cells transfected with hCXCR-4 and human T-lymphocytes cultured for 5 - 10 days. The expected ED<sub>50</sub> for these effects are typically 1-5 ng/ml and 10-30 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
kpvslsyrc pcrffeshva ranvkhlkil ntpncalqiv arlknnnrqv cidpklkwiq eylekalnkr fkm
N-terminal Sequence Analysis
Lys22
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human SDF-1β is Mr 8 kDa.
Predicted Molecular Mass
8
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Muller, HW. et al. (2000) Eur J Neurosci. 12: 1857
2. Nagai, Y. et al. (1998) Proceedings of National Acad Sci USA 95: 6331
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
S115
S110
Products are for research use only. Not for use in diagnostic or therapeutic procedures.