Pricing & Details
Purified Recombinant Human SDF-1 β (>98%)
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 10 ng/mL Recombinant Human/Feline CXCL12/SDF‑1 beta.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human Stromal Cell-Derived Factor-1 Beta (SDF-1 β) recognizes Human SDF-1 β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
SDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.1 SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.2
NCBI Gene Bank ID
References & Citations
1. Muller, HW. et al. (2000) Eur J Neurosci. 12:1857
2. Nagai, Y. et al. (1998) Proceedings of National Acad Sci USA 95:6331
Products are for research use only. Not for use in diagnostic or therapeutic procedures.