Recombinant Human Sonic Hedgehog (C24II) N-Terminus

Sonic hedgehog (SHH) is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb.¹ SSH functions as a secreted signaling molecule in limb pattern formation, differentiation of motor neurons, and sclerotome induction.² SHH pathway is known to be functional in the developing male tract.³ SHH may have therapeutic value as a protective agent in neurodegenerative disease.⁴

The biological activity of recombinant Human Sonic Hedgehog is determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED₅₀ is 0.1-0.4 μg/ml.

The DNA sequence encoding amino acid residues Cys 24 - Gly 197 of human SHH with Ile-Ile substituted for Cys 24 (Riddle, R.D. et al., 1993, Cell 75:1401 - 1416; Taylor, F.R. et al., 2001, Biochemistry 40:4359 - 4371) was inserted into a suitable expression vector and expressed in E. coli.

Lyophilized

The predicted molecular weight of Recombinant Human SHH is Mr 20 kDa.

20

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with 0.3 M NaCl, no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Tabin, CJ. et al. (1996) Dev Biol. 180: 2732. Noji S et al. (1996) Biochem Biophys Res Commun 216: 68
3. Jacobs, JP. et al. (2004) J Androl. 25: 514
4. Pang, K. et al. (1997) J Neurosci. 17: 5891