Recombinant Human Sonic Hedgehog (C24II) N-Terminus

Recombinant Human Sonic Hedgehog (C24II) N-Terminus

Product No.: S561

[product_table name="All Top" skus="S561"]

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Alternate Names
HHG1, HLP3, HPE3, SMMCI
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Background

Sonic hedgehog (SHH) is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb.1 SSH functions as a secreted signaling molecule in limb pattern formation, differentiation of motor neurons, and sclerotome induction.2 SHH pathway is known to be functional in the developing male tract.3 SHH may have therapeutic value as a protective agent in neurodegenerative disease.4

Protein Details

Purity
>95% by SDS Page and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of recombinant Human Sonic Hedgehog is determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED<sub>50</sub> is 0.1-0.4 μg/ml.
Amino Acid Sequence
The DNA sequence encoding amino acid residues Cys 24 - Gly 197 of human SHH with Ile-Ile substituted for Cys 24 (Riddle, R.D. et al., 1993, Cell 75:1401 - 1416; Taylor, F.R. et al., 2001, Biochemistry 40:4359 - 4371) was inserted into a suitable expression vector and expressed in E. coli.
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human SHH is Mr 20 kDa.
Predicted Molecular Mass
20
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with 0.3 M NaCl, no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient

References & Citations

1. Tabin, CJ. et al. (1996) Dev Biol. 180: 2732. Noji S et al. (1996) Biochem Biophys Res Commun 216: 68
3. Jacobs, JP. et al. (2004) J Androl. 25: 514
4. Pang, K. et al. (1997) J Neurosci. 17: 5891
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.