Recombinant Mouse Sonic Hedgehog (N Terminus)

Recombinant Mouse Sonic Hedgehog (N Terminus)

Product No.: S573

[product_table name="All Top" skus="S573"]

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Alternate Names
HHG1, HLP3, HPE3, SMMCI, Hx, Dsh, Hxl3, M100081, 9530036O11Rik
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Mouse

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Data

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Background

Sonic hedgehog (Shh) is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb.1 Shh functions as a secreted signaling molecule in limb pattern formation, differentiation of motor neurons, and sclerotome induction.2 Shh pathway is known to be functional in the developing male tract.3 Shh may have therapeutic value as a protective agent in neurodegenerative disease.4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse Sonic Hedge Hedgehog was determined by its ability to induce ALK Phos production by C3H10T1/2 mouse fibroblasts. The expected ED<sub>50</sub>=0.6 - 3.0 µg/ml.
Fusion Protein Tag
Histidine Tag
Protein Accession No.
Amino Acid Sequence
cgpgrg fgkrrhpkkl tplaykqfip nvaektlgas gryegkitrn serfkeltpn ynpdiifkde entgadrlmt qrckdklnal aisvmnqwpg vklrvtegwd edghhseesl hyegravdit tsdrdrskyg mlarlaveag fdwvyyeska hihcsvkaen svaaksgghh hhhh
N-terminal Sequence Analysis
Cys25
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse Shh is Mr 20 kDa.
Predicted Molecular Mass
20
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Tabin, CJ. et al. (1996) Dev Biol. 180: 273
2. Noji, S. et al. (1996) Biochem Biophys Res Commun 216: 68
3. Jacobs, JP. et al. (2004) J Androl. 25: 514
4. Pang, K. et al. (1997) J Neurosci. 17: 5891
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.