Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1
>97% by SDS-PAGE and analyzed by silver stain.
<1.0 EU/µg as determined by the LAL method
The biological activity of Human Thrombopoietin is determined by the dose-dependent stimulation of the proliferation of human MO7e cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
spappacdl rvlskllrds hvlhsrlsqc pevhplptpv llpavdfslg ewktqmeetk aqdilgavtl llegvmaarg qlgptclssl lgqlsgqvrl llgalqsllg tqlppqgrtt ahkdpnaifl sfqhllrgkv rflmlvggst lcvrrapptt avpsrtslvl tlnelpnrts glletnftas arttgsgllk wqqgfrakip gllnqtsrsl dqipgylnri hellngtrgl fpgpsrrtlg apdissgtsd tgslppnlqp gyspspthpp tgqytlfplp ptlptpvvql hpllpdpsap tptptsplln tsythsqnls qeg
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 43-60 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 20% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda, RC. et al. (1998) Blood 92: 4023
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.