Anti-Human TPO

Pricing & Details

Product No.T148
Protein
TPO
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Thrombopoietin, THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T148-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
T148-100 µg
100 µg
$442.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human TPO (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 10 ng/mL Recombinant Human Thrombopoietin/Tpo.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Thrombopoietin (TPO) recognizes Human TPO. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Antigen Distribution
Human TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle.
Background
Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda, RC. et al. (1998) Blood 92: 4023
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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