Recombinant Human WISP-1

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Pricing & Details

Product. No.W105
Alternate Names
CCN4, WISP1c, WISP1i, WISP1tc
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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W105-50 µg
50 µg
$359.00
In stock
Max:
Min: 1
Step: 1

Background

WNT1 inducible signaling pathway protein 1 (WISP-1), also known as CCN4, is a secreted, heparin-binding glycoprotein belonging to the cysteine-rich CCN family (1-2). WISP-1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. It is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival (3). WISP-1 promotes mesenchymal cell proliferation and osteoblastic differentiation while repressing chondrocytic differentiation. Therefore, WISP-1 plays an important regulatory role during bone development and fracture repair (4). It was reported that WISP-1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation (5). WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein (5). Mutated or spliced variants of WISP-1 have been implicated in various pathological conditions.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED50 for this effect is 1-6 µg/mL.
Fusion Protein Tag
Histidine Tag
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
talspapt tmdftpaple dtssrpqfck wpcecppspp rcplgvslit dgcecckmca qqlgdnctea aicdphrgly cdysgdrpry aigvcaqvvg vgcvldgvry nngqsfqpnc kynctcidga vgctplclrv rpprlwcphp rrvsipghcc eqwvceddak rprktaprdt gafdavgeve awhrnciayt spwspcstsc glgvstrisn vnaqcwpeqe srlcnlrpcd vdihtlikag kkclavyqpe asmnftlagc istrsyqpky cgvcmdnrcc ipyksktidv sfqcpdglgf srqvlwinac fcnlscrnpn difadlesyp dfseianhhh hhhhhhh
N-terminal Sequence Analysis
Thr23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human WISP-1 is Mr 39.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 62 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Tchou-Wong, KM. et al. (2003) J. Bio.l Chem. 278:11465
2. Levine, AJ. et al. (2002) Genes Dev. 16:46
3. Pennica, D. et al. (1998) Proc. Nat. Acad. Sci. (USA) 95:14717
4. Desnoyers, L. et al. (2004) Am. J. Pathol. 165:855
5. Levine, AJ. et al. (2000) Genes Dev. 14:585
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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