Recombinant Human WISP-1

WNT1 inducible signaling pathway protein 1 (WISP-1), also known as CCN4, is a secreted, heparin-binding glycoprotein belonging to the cysteine-rich CCN family (1-2). WISP-1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. It is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival (3). WISP-1 promotes mesenchymal cell proliferation and osteoblastic differentiation while repressing chondrocytic differentiation. Therefore, WISP-1 plays an important regulatory role during bone development and fracture repair (4). It was reported that WISP-1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation (5). WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein (5). Mutated or spliced variants of WISP-1 have been implicated in various pathological conditions.

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED₅₀ for this effect is 1-6 µg/mL.

Histidine Tag

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Thr23

Lyophilized

The predicted molecular weight of Recombinant Human WISP-1 is Mr 39.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 62 kDa.

39.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Tchou-Wong, KM. et al. (2003) J. Bio.l Chem. 278:11465
2. Levine, AJ. et al. (2002) Genes Dev. 16:46
3. Pennica, D. et al. (1998) Proc. Nat. Acad. Sci. (USA) 95:14717
4. Desnoyers, L. et al. (2004) Am. J. Pathol. 165:855
5. Levine, AJ. et al. (2000) Genes Dev. 14:585