Recombinant Mouse Adiponectin

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Pricing & Details

Product. No.A411
Alternate Names
APN, Acdc, Apm1, 30kDa, GBP28, Adipo, Adipoq
Expression Host
NS0 cells
Species
Mouse
Applications
ELISA Cap
FC
Prod No.
Size
Price
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Qty
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A411-50 µg
50 µg
$410.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
edd vttteelapa lvpppkgtca gwmagipghp ghngtpgrdg rdgtpgekge kgdagllgpk getgdvgmtg aegprgfpgt pgrkgepgea aymyrsafsv gletrvtvpn vpirftkify nqqnhydgst gkfycnipgl yyfsyhitvy mkdvkvslfk kdkavlftyd qyqeknvdqa sgsvllhlev gdqvwlqvyg dgdhnglyad nvndstftgf llyhdtnhhh hhh
N-terminal Sequence Analysis
Glu18
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse Acrp30 is Mr 25.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-38 kDa.
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six months at 2 to 8°C as supplied. Do Not Freeze.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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