Recombinant Mouse FGF-Acidic

Fibroblast growth factor 1 (acidic), also known as FGF1, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
FGF-1 is the only member of the FGF family that binds with high affinity to all four FGF receptors.¹

FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.²

The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/ml corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.

MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Jaye, M. et al. (1986) Science 233:541
2. Otlewski, J. et al. (2009) Acta. Crystallogr. D. Biol. Crystallogr. 65:67
3. Nakazawa, F. et al. (2006) Blood. 108:3335
4. Bjornsson, TD. et al. (1991) Proc. Natl. Acad. Sci. (USA) 88:8651
5. Merwin, JR. et al. (1992) Cancer Res. 52:4995