Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells1 , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.2 IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.3
>97% by SDS-PAGE and analyzed by silver stain.
<1.0 EU/µg as determined by the LAL method
The biological activity of Mouse IL-12 was determined in a proliferation assay using PHA-activated mouse splenocytes. The expected ED50= 0.01 - 0.1 ng/ml.
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
mwelekdvyv vevdwtpdap getvnltcdt peedditwts dqrhgvigsg ktltitvkef ldagqytchk ggetlshshl llhkkengiw steilknfkn ktflkceapn ysgrftcswl vqrnmdlkfn ikssssspds ravtcgmasl saekvtldqr dyekysvscq edvtcptaee tlpielalea rqqnkyenys tsffirdiik pdppknlqmk plknsqvevs weypdswstp hsyfslkffv riqrkkekmk eteegcnqkg aflvektste vqckggnvcv qaqdryynss cskwacvpcr vrsrvipvsg parclsqsrn llkttddmvk tareklkhys ctaedidhed itrdqtstlk tclplelhkn esclatrets sttrgsclpp qktslmmtlc lgsiyedlkm yqtefqaina alqnhnhqqi ildkgmlvai delmqslnhn getlrqkppv geadpyrvkm klcillhafs trvvtinrvm gylssa
N-terminal Seqence Analysis
Met23 (p40) & Arg23 (p35)
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-12 is 70 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 25 kDa and 43 kDa (reducing conditions) and 68 kDa (non-reducing conditions).
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28
2. Ritz, J. et al. (2001) Blood. 97(12):3860-6.
3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.