CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
The predicted molecular weight of Recombinant Mouse IL-7 Rα is Mr 51.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
Predicted Molecular Mass
51.6
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
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Recombinant Mouse IL-7 Rα (Interleukin-7 Receptor alpha) is a critical reagent for research focused on immune cell development, homeostasis, and signaling, particularly in mouse models. Its use enables precise investigation of IL-7/IL-7Rα-mediated pathways, which are essential for T and B lymphocyte biology, immune regulation, and disease modeling.
Key reasons to use recombinant Mouse IL-7 Rα in research applications:
Dissection of IL-7 Signaling Pathways: IL-7Rα is the primary receptor subunit mediating IL-7 signaling, which is crucial for the survival, proliferation, and differentiation of T and B cells. Recombinant IL-7 Rα allows for controlled in vitro and in vivo studies of these pathways, including receptor-ligand binding, downstream STAT5 phosphorylation, and gene transcription.
Modeling Immune Cell Development and Homeostasis: IL-7Rα is indispensable for normal lymphocyte development and maintenance. Using recombinant IL-7 Rα, researchers can study how IL-7 signaling influences the expansion of pro-B and pre-B cells, as well as the survival of naïve and memory T cells.
Investigation of Immune Dysregulation and Disease: Aberrant IL-7/IL-7Rα signaling is implicated in autoimmune diseases, inflammatory conditions, and cancer. Recombinant IL-7 Rα can be used to model these diseases, test the effects of receptor blockade or stimulation, and evaluate therapeutic interventions.
Therapeutic Target Validation: IL-7Rα is a target for immunomodulatory therapies. Recombinant protein enables screening of antibodies, small molecules, or biologics that modulate IL-7Rα function, supporting drug discovery and mechanistic studies.
Functional Assays and Receptor Binding Studies: Recombinant IL-7 Rα is used in binding assays to characterize ligand specificity, affinity, and downstream signaling events, as well as to generate standard curves for quantifying IL-7 or IL-7Rα in biological samples.
Tool for Blocking or Mimicking IL-7Rα Activity: Recombinant IL-7 Rα can act as a decoy receptor to sequester IL-7, or as a competitor in receptor-ligand interaction studies, providing a means to modulate IL-7 signaling experimentally.
Applications include:
In vitro T and B cell culture systems to study proliferation, survival, and differentiation.
In vivo mouse models of lymphopenia, autoimmunity, or cancer to assess the impact of altered IL-7Rα signaling.
Screening and validation of therapeutic antibodies or inhibitors targeting IL-7Rα.
Mechanistic studies of cytokine signaling and immune regulation.
In summary, recombinant Mouse IL-7 Rα is an essential tool for immunology research, enabling detailed analysis of IL-7-driven processes in mouse systems and supporting the development of targeted immunotherapies.
Recombinant Mouse IL-7 Rα (CD127) protein is generally not suitable as a standard for quantification or calibration in ELISA assays designed to measure IL-7 cytokine concentrations. ELISA standards must match the analyte being quantified—in this case, mouse IL-7, not its receptor.
Key context and supporting details:
ELISA standards are typically recombinant or purified forms of the target analyte (e.g., mouse IL-7 protein) and are used to generate a standard curve for quantification of that specific molecule in biological samples.
IL-7 Rα (CD127) is the receptor for IL-7, not the cytokine itself. Its structure, immunoreactivity, and detection characteristics differ from IL-7. Using IL-7 Rα as a standard would not accurately reflect the concentration of IL-7 in your samples, as the antibodies in IL-7 ELISA kits are specific for IL-7, not its receptor.
ELISA kits for IL-7 are validated to detect and quantify mouse IL-7, using recombinant mouse IL-7 as the standard. These kits demonstrate parallelism and recovery with both recombinant and natural IL-7, ensuring accurate quantification.
Recombinant proteins for ELISA standards must be validated for use as standards in the specific assay. Recombinant IL-7 Rα may be used as a standard only in ELISA kits specifically designed to quantify IL-7 Rα (CD127), not IL-7.
Best practices:
Use recombinant mouse IL-7 as the standard for IL-7 quantification ELISAs.
Use recombinant mouse IL-7 Rα only as a standard in ELISAs specifically designed to measure IL-7 Rα (CD127) concentrations.
Summary Table:
ELISA Target
Appropriate Standard
Not Appropriate Standard
Mouse IL-7
Recombinant Mouse IL-7
Recombinant Mouse IL-7 Rα
Mouse IL-7 Rα
Recombinant Mouse IL-7 Rα
Recombinant Mouse IL-7
If your goal is to quantify IL-7 Rα (CD127), use an ELISA kit validated for that target and use recombinant IL-7 Rα as the standard. For IL-7 quantification, use recombinant IL-7 as the standard.
Recombinant Mouse IL-7 Rα has been validated for several key applications in published research, primarily in studies of immune cell signaling, autoimmune disease models, cancer immunotherapy, and cell biology.
Validated Applications:
Binding and Affinity Assays: Recombinant mouse IL-7 Rα is frequently used in in vitro binding assays to characterize the interaction with IL-7 and engineered IL-7 variants. For example, yeast display and quantitative binding assays have been used to measure the affinity of IL-7 and its mutants for mouse IL-7 Rα, providing dissociation constants and informing protein engineering efforts.
Functional Blocking Studies: Anti-IL-7Rα antibodies (targeting recombinant mouse IL-7 Rα) are used to block IL-7 signaling in in vivo models, such as autoimmune disease (e.g., alopecia areata, rheumatoid arthritis). These studies assess the impact of IL-7Rα blockade on T cell infiltration, cytokine production, and disease severity.
Cellular Proliferation and Survival Assays: Recombinant IL-7 and IL-7Rα are used to study their effects on lymphocyte proliferation, survival, and differentiation, particularly in T cell and B cell development. These assays include flow cytometry, cell counting, and analysis of signaling pathways (e.g., STAT5 phosphorylation).
Immunotherapy and Disease Models: Recombinant mouse IL-7 Rα is used in preclinical models to evaluate the therapeutic potential of IL-7 pathway modulation in cancer, chronic viral infection, and lymphopenia. These studies involve administration of recombinant proteins or antibodies and monitoring immune cell populations, tumor growth, and viral clearance.
Protein Characterization: Recombinant mouse IL-7 Rα is validated for use in protein characterization techniques such as SDS-PAGE, HPLC, functional assays, and mass spectrometry to confirm purity, activity, and molecular weight.
Summary Table of Validated Applications
Application Type
Example Techniques/Models
Reference(s)
Binding/Affinity Assays
Yeast display, quantitative binding
Functional Blocking
Autoimmune models, antibody blockade
Cellular Proliferation/Survival
Flow cytometry, signaling assays
Immunotherapy/Disease Models
Cancer, viral infection, lymphopenia
Protein Characterization
SDS-PAGE, HPLC, mass spectrometry
Additional Notes:
Recombinant mouse IL-7 Rα is essential for dissecting the molecular mechanisms of IL-7 signaling in both in vitro and in vivo systems.
It is widely used in combination with recombinant IL-7 or engineered IL-7 variants to study receptor-ligand interactions and downstream immune effects.
Blocking or modulating IL-7Rα function is a validated strategy in models of autoimmunity, cancer, and immune deficiency.
If you require protocol details or specific assay recommendations for recombinant mouse IL-7 Rα, please specify the intended application.
To reconstitute and prepare Recombinant Mouse IL-7 Rα protein for cell culture experiments, use sterile, endotoxin-free conditions and follow these steps:
Equilibrate the vial: Allow the lyophilized protein to reach room temperature before opening to minimize condensation.
Centrifuge briefly: Spin down the vial to collect the powder at the bottom before opening.
Reconstitution buffer:
Most protocols recommend using sterile distilled water or sterile PBS (pH 7.4) for reconstitution.
Some protocols suggest 10 mM acetic acid for initial solubilization, especially if the protein is difficult to dissolve.
Avoid reconstituting at concentrations above 1 mg/mL to prevent aggregation.
Reconstitution concentration:
Typical concentrations for reconstitution are 0.1–0.5 mg/mL.
For example, dissolve a 5 µg vial in 100 µL sterile PBS to achieve 50 µg/mL.
Mix gently: Resuspend by gentle pipetting along the vial wall. Do not vortex to avoid protein denaturation.
Aliquot and storage:
After reconstitution, aliquot to avoid repeated freeze-thaw cycles.
Store aliquots at –20°C to –80°C for long-term use, or at 4°C for short-term (2–7 days).
For extended storage, dilute in buffer containing 0.1% BSA to stabilize the protein.
Sterility: Ensure all solutions and containers are sterile to prevent contamination in cell culture.
Preparation for cell culture experiments:
Dilute the reconstituted stock to the desired working concentration using sterile cell culture medium or buffer immediately before use.
Typical working concentrations for functional assays range from 1–100 ng/mL, but optimal dosing should be determined empirically for your cell type and assay.
Additional notes:
If the protein is difficult to dissolve, low pH buffers (e.g., acetic acid) may improve solubility, but always dilute into neutral pH buffer before adding to cells.
Avoid repeated freeze-thaw cycles, which can reduce bioactivity.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
