Recombinant Mouse MIP-1β
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BackgroundBoth MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse MIP-1β was determined by its ability to chemoattract mouse BaF/3 cells transfected with human CCR5. . The expected ED<sub>50</sub> for this effect is typically 3 - 12 ng/ml. Protein Accession No. Q5QNV9 Amino Acid Sequence apmgsdppts ccfsytsrql hrsfvmdyye tsslcskpav vfltkrgrqi canpsepwvt eymsdleln
N-terminal Sequence Analysis Ala24 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse MIP-1β is Mr 7.8 kDa. Predicted Molecular Mass 7.8 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Sun, X. et al. (2006) Infec. Immun. 74:5943. Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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