Name: Anti-Human MIP-1β (Clone 24006)
SKU: M1270
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Anti-Human MIP-1β (Clone 24006)

Product No.: M1270


Clone 24006 Protein MIP-1 beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL4, ACT2, AT744.1, G-26, LAG1, MGC104418, MGC126025, MGC126026, MIP1B1, SCYA2, SCYA4 Isotype Mouse IgG2b Applications CyTOF® , ELISA Cap , FC , N , WB


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Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human MIP-1β (Accession # P13236)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831264

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:M193 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:M162.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-1β. A suggested method would be to stain cells expressing MIP-1β with 0.25-0.50 µg per 1-5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human MIP-1β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M162.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human MIP-1β bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human MIP-1β (at 0.04 ng/ml) is 0.3 - 1.2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human MIP-1β/CCL4 (Clone 24006) recognizes an epitope on Human MIP-1β/CCL4. In Western blots, this antibody shows less than 4% crossreactivity with recombinant human (rh) CCL3/MIP1α and does not crossreact with recombinant mouse (rm) CCL3/MIP1α or rmCCL4/MIP1β. This monoclonal antibody was purified using affinity chromatography methods

Background

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.