Anti-Human MIP-1β (Clone 24006)

Pricing & Details

Product No.M1270
Clone
24006
Protein
MIP-1 beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL4, ACT2, AT744.1, G-26, LAG1, MGC104418, MGC126025, MGC126026, MIP1B1, SCYA2, SCYA4
Isotype
Mouse
IgG2b
Applications
CyTOF®
,
ELISA Cap
,
FC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1270-25 µg
25 µg
$230.00
In stock
Max:
Min: 1
Step: 1
M1270-100 µg
100 µg
$424.00
In stock
Max:
Min: 1
Step: 1
M1270-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MIP-1β (Accession # P13236)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:M193 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:M162.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-1β. A suggested method would be to stain cells expressing MIP-1β with 0.25-0.50 µg per 1-5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human MIP-1β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M162.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MIP-1β bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIP-1β (at 0.04 ng/ml) is 0.3 - 1.2 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human MIP-1β/CCL4 (Clone 24006) recognizes an epitope on Human MIP-1β/CCL4. In Western blots, this antibody shows less than 4% cross­reactivity with recombinant human (rh) CCL3/MIP­1α and does not cross­react with recombinant mouse (rm) CCL3/MIP­1α or rmCCL4/MIP­1β. This monoclonal antibody was purified using affinity chromatography methods
Background
Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.

Antigen Details

Protein
Elisa Sandwich Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
M1270
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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