Recombinant Mouse MMP-3

The specific activity, measured with 10 μM ES002 and 50 ng enzyme in 100 μL of TCNB (50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5) at room temperature, is > 300 pmol/min/μg.

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Tyr18

Solution

The predicted molecular weight of Recombinant Mouse MMP-3 is Mr 52 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 60 kDa.

52

Supplied frozen as a 0.2 μm filtered solution containing 25 mM Tris, 5 mM CaCl₂, 50 mM NaCl, 0.02% Brij-35, 50% Glycerol, pH 8.0 at a concentration of 0.50 mg/ml.

This protein is stable for one month when stored sterile at -20°C or for three months when stored at -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Polar Packs

1. Tang, CH. et al. (2011) J Cell Biochem. 112(5):1431-40.
2 Tang, CH. et al. (2012) J Cell Biochem. 113(6):1977-86.