Anti-Mouse MMP-3
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0 - Derived Recombinant Mouse MMP-3 Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831289 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MMP-3 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limitfor rmMMP-3 is approximately 5 ng/lane and 25 ng/lane under non-reducingand reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunoprecipitation: This antibody has been used at 25 μg/mL to immunoprecipitate rmMMP-3 from conditioned media of transfected NS0 cells. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Matrix Metalloproteinase 3 (MMP-3) recognizes Mouse MMP-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
