Neurotrophin 3 (NT-3), also known as NGF-2 and HDNF, is a basic protein and member of the NGF family. It is structurally related to β-NGF, BDNF and NT-4 (1), is expressed by neurons of the central nervous systems and can signal through the Trk receptors (2). NT-3 is also expressed in muscles and its expression is down-regulated in denervated muscles. It rapidly potentiates the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses (3). NT-3 negatively modulates NGF receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. In addition, NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI) (4). NT-3 affects development of neurons in the embryo when it is expressed in human placenta. Mice born without the ability to make NT-3 show the loss of pro-prioceptive including subsets of mechanoreceptive sensory neurons (5). The amino acid sequences of human, murine and rat NT-3 are identical.
<0.1 EU/µg as determined by the LAL method
The biological activity of Mouse NT-3 was determined by the dose-dependent proliferation of BaF3 cells transfected with the TrkB receptor. The expected ED50= 1-10 ng/ml.
Protein Accession No.
Amino Acid Sequence
YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse NT-3 is Mr 13.6 kDa.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Barde, YA. et al. (1990) Prog. Growth Factor Res. 2:237
2. Tessarollo, L. et al. (1993) Development 118:463
3. Maisonpierre, PC. et al. (1990) Science 247:1446
4. Valerie, MK. et al. (2005) J. Neuroscie. 25:758
5. Tessarollo, L. et al. (1994) Proc. Natl. Acad. Sci. (USA) 91:11844
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.