Recombinant Mouse PDGF-BB

Recombinant Mouse PDGF-BB (Platelet-Derived Growth Factor-BB) is a valuable tool for a wide range of research applications due to its well-characterized biological activities and reproducible performance. Here are several key reasons to consider using recombinant mouse PDGF-BB in your research:

1. Stimulates Mesenchymal Stem Cell (MSC) Proliferation

Recombinant mouse PDGF-BB has been shown to significantly enhance the proliferation of mesenchymal stem cells (MSCs) both in vitro and in vivo. Studies indicate that PDGF-BB at concentrations such as 3–30 ng/mL can robustly increase MSC expansion, making it ideal for experiments involving stem cell culture, differentiation, and regenerative medicine research.

2. Promotes Angiogenesis

PDGF-BB plays a critical role in promoting angiogenesis—the formation of new blood vessels. In vivo studies have demonstrated increased vascularization in tissues treated with PDGF-BB, as evidenced by higher numbers of small vessels in the marrow cavity and upregulation of endothelial markers. This makes it useful for research on tissue regeneration, wound healing, and vascular biology.

3. Enhances Bone Formation and Osteoblastogenesis

PDGF-BB, at optimal doses, synergizes with other osteogenic factors (such as BMP2) to promote osteoblast differentiation and bone formation. Research shows that PDGF-BB can increase bone mineral density and trabecular bone formation, especially when used at controlled concentrations. This is relevant for studies on bone regeneration, osteoporosis, and skeletal tissue engineering.

4. Supports Platelet Recovery and Hematopoiesis

In models of radiation-induced myelosuppression, recombinant PDGF-BB has demonstrated thrombopoietic effects, supporting platelet recovery. This makes it a useful reagent for studies on hematopoiesis, bone marrow transplantation, and recovery from cytopenia.

5. Consistent and Reproducible Activity

Recombinant mouse PDGF-BB is produced under controlled conditions, ensuring batch-to-batch consistency and high biological activity. This is essential for reliable and reproducible experimental results, especially in quantitative assays and comparative studies.

6. Versatile Applications

Recombinant mouse PDGF-BB can be used in a variety of experimental systems, including:

• Cell culture and expansion of MSCs and other progenitor cells
• Functional assays for proliferation, migration, and differentiation
• In vivo models of tissue repair, bone regeneration, and wound healing
• Studies on vascular biology and angiogenesis

7. Well-Characterized and Widely Cited

The biological effects of PDGF-BB have been extensively studied and documented in peer-reviewed literature, providing a strong scientific foundation for its use in research. Its role in tissue regeneration, stem cell biology, and wound healing is well established.

8. Optimized for Mouse Models

Using recombinant mouse PDGF-BB ensures species-specific compatibility in mouse models, minimizing potential issues related to cross-species reactivity and maximizing biological relevance.

In summary, recombinant mouse PDGF-BB is a powerful and versatile growth factor that supports a broad range of research applications, particularly in stem cell biology, tissue regeneration, bone formation, and vascular biology. Its well-documented effects, reproducible activity, and compatibility with mouse models make it an excellent choice for experimental studies.

Yes, recombinant Mouse PDGF-BB can be used as a standard for quantification or calibration in ELISA assays, provided it is compatible with the antibodies and assay format of your specific ELISA kit. Recombinant proteins are commonly used as standards in quantitative ELISA protocols to generate standard curves for accurate measurement of target analyte concentrations.

Key considerations for using recombinant Mouse PDGF-BB as an ELISA standard:

• Assay Compatibility: Ensure the recombinant Mouse PDGF-BB is recognized by the capture and detection antibodies in your ELISA kit. Most mouse PDGF-BB ELISA kits are designed to detect both natural and recombinant forms, and manufacturers routinely use recombinant PDGF-BB for calibration.
• Purity and Source: The recombinant standard should be highly purified and preferably produced in a system (e.g., E. coli, mammalian cells) that yields a protein with similar post-translational modifications to the native protein, if relevant for antibody recognition.
• Standard Curve Preparation: Prepare serial dilutions of the recombinant PDGF-BB in the same buffer or matrix as your samples to minimize matrix effects and ensure accurate quantification.
• Validation: Confirm that the standard curve generated with recombinant PDGF-BB is linear and parallel to curves obtained with natural PDGF-BB, if available. Most validated ELISA kits report parallelism between recombinant and natural PDGF-BB, supporting its use for quantification.
• Documentation: Follow the ELISA kit protocol for standard preparation and calibration. Kits typically specify the concentration range and dilution steps for the standard curve.

Best Practices:

• Use the recombinant Mouse PDGF-BB within the recommended concentration range and storage conditions to maintain stability and accuracy.
• Validate recovery and linearity in your sample matrix by spiking known amounts of recombinant PDGF-BB and assessing recovery rates.

Limitations:

• If your ELISA kit is specific for human PDGF-BB, recombinant mouse PDGF-BB should not be used as a standard due to species-specific antibody recognition.
• Always consult the technical datasheet of your ELISA kit to confirm compatibility with recombinant standards.

In summary, recombinant Mouse PDGF-BB is suitable and routinely used as a standard for calibration in mouse PDGF-BB ELISA assays, provided it matches the specificity requirements of your kit and is properly validated.

Recombinant Mouse PDGF-BB has been validated for a range of applications in published research, primarily in the context of cell biology, tissue regeneration, and disease models. The most commonly validated applications include:

• Functional assays: Used to assess cell proliferation, migration, and survival, particularly in mesenchymal cells, fibroblasts, vascular smooth muscle cells, and glial cells.
• ELISA (Enzyme-Linked Immunosorbent Assay): For quantifying PDGF-BB levels or activity in biological samples.
• Western blot: For detecting PDGF-BB protein expression in cell and tissue lysates.
• Immunohistochemistry: For localizing PDGF-BB in tissue sections.

Published research applications include:

• Wound healing and tissue regeneration: Recombinant mouse PDGF-BB is widely used to study and promote wound healing, including in diabetic and chronic wound models, as well as in regeneration of oral/maxillofacial tissues and bone.
• Angiogenesis and arteriogenesis: Validated in studies inducing new blood vessel formation and maturation, especially in diabetic mouse skin and ischemic tissues.
• Vascular biology and blood-brain barrier studies: Used to investigate vascular repair, pericyte recruitment, and maintenance of blood-brain barrier integrity in mouse models.
• Sepsis and inflammation models: Demonstrated to improve survival and reduce tissue damage in mouse models of sepsis by modulating inflammatory cytokine production.
• Cell signaling and developmental biology: Applied in studies of embryonic development, organogenesis, and skeletal development.
• Fibroblast activation and fibrosis: Used to study fibroblast activation protein (FAP) regulation and vascular smooth muscle cell behavior.

Experimental protocols often involve:

• Direct application to cell cultures or tissue explants for proliferation/migration assays.
• Topical or intradermal delivery in animal models for wound healing and angiogenesis studies.
• Intravenous or local injection in disease models (e.g., sepsis, ischemia).

Summary Table: Key Validated Applications

These applications are supported by both product validation data and peer-reviewed research, confirming the utility of recombinant mouse PDGF-BB in diverse experimental systems.

To reconstitute and prepare Recombinant Mouse PDGF-BB protein for cell culture experiments, follow these best-practice steps:

• Centrifuge the vial briefly before opening to ensure all lyophilized protein is at the bottom.
• Reconstitute the lyophilized protein in 100 mM acetic acid containing 0.1% BSA (bovine serum albumin) to a final concentration of 0.1–1.0 mg/mL. This helps regain full biological activity and prevents protein loss due to adsorption.
• Mix gently to ensure complete dissolution. Allow several minutes for full reconstitution.
• Aliquot the reconstituted protein into working volumes to avoid repeated freeze-thaw cycles, which can reduce activity.
• Store aliquots at ≤ –20°C for long-term storage. For short-term use (2–7 days), storage at 4–8°C is acceptable.
• For further dilutions (e.g., to working concentrations for cell culture), use low endotoxin medium or a buffered solution containing FBS or tissue culture grade BSA. This maintains protein stability and minimizes endotoxin exposure to cells.
• Avoid reconstituting to concentrations below 100 μg/mL to maintain protein stability.

Summary of key steps:

1. Briefly centrifuge vial.
2. Add 100 mM acetic acid + 0.1% BSA to reach 0.1–1.0 mg/mL.
3. Mix gently, allow to dissolve.
4. Aliquot and store at ≤ –20°C.
5. Dilute to working concentration in cell culture medium with serum or BSA.

Additional notes:

• The optimal working concentration for cell culture should be empirically determined for your specific assay, but typical effective concentrations range from 1–100 ng/mL depending on cell type and application.
• Always use sterile technique and low endotoxin reagents to avoid contamination and ensure reproducibility.

This protocol ensures maximal activity and stability of recombinant mouse PDGF-BB for cell culture applications.