Recombinant Mouse PDGF R-β

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Pricing & Details

Product. No.P248
Alternate Names
Platelet-Derived Growth Factor Receptor Beta, TEL, PDGFRB, FLJ12858, PDGF2, SIS, SSV, C-sis, CD140B, JTK12, PDGFR, PDGFR1
Expression Host
NS0 cells
Species
Mouse
Prod No.
Size
Price
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Qty
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P248-100 µg
100 µg
$379.00
In stock
Max:
Min: 1
Step: 1

Background

Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRB and PDGF-R-beta, is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, that form disulfide-linked homodimers and a heterodimer (1). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two tyroine kinase receptors (PDGF Rα and Rβ) (2). These two receptor isoforms dimerize upon binding PDGF leading to three possible receptor combinations, namely -αα, -ββ and -αβ. The extracellular region of the PDGF receptor-β consists of five immunoglobulin-like domains and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans leading to the activation of several intracellular pathways, such as MAPK, PI3K and PLCy (3). Many cell types, including fibroblasts and smooth muscle cells express both α and β receptors while others only express one (4).

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse PDGFR-β was determined by its ability to inhibit the PDGF-BB induced 3H-Thymidine incorporation in the NR6R-3T3 fibroblast cell-line (Raines, E.W.et al., 1985, Methods Enzymol. 109:749). The expected ED50 for this effect is typically 0.02 – 0.05 μg/mL in the presence of 4 ng/mL rhPDGF-BB.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
lvitppgpe fvlnisstfv ltcsgsapvm weqmsqvpwq eaamnqdgtf ssvltltnvt ggdtgeyfcv ynnslgpels erkriyifvp dptmgflpmd sedlfifvtd vtettipcrv tdpqlevtlh ekkvdiplhv pydhqrgftg tfedktyick ttigdrevds dtyyvyslqv ssinvsvnav qtvvrqgesi tircivmgnd vvnfqwtypr mksgrlvepv tdylfgvpsr igsilhipta elsdsgtytc nvsvsvndhg dekainisvi engyvrllet lgdveiaelh rsrtlrvvfe aypmpsvlwl kdnrtlgdsg agelvlstrn msetryvsel ilvrvkvsea gyytmrafhe ddevqlsfkl qvnvpvrvle lseshpange qtircrgrgm pqpnvtwstc rdlkrcprkl sptplgnssk eesqletnvt fweedqeyev vstlrlrhvd qplsvrcmlq nsmggdsqev tvvphslpfk iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk
N-terminal Sequence Analysis
Leu32
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse PDGF Rβ is Mr 82.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 124 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512
2. Arcaro, A. et al. (2000) Mol. Cell. Biol. 20:3817
3. Chi, KD. et al. (1997) Oncogene 15:1051
4. Morerio, C. et al. (2004) Cancer Res. 64:264
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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