Anti-Human PDGF Rβ
Anti-Human PDGF Rβ
Product No.: P268
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Target PDGF R beta Product Type Polyclonal Antibody Alternate Names Platelet-Derived Growth Factor Receptor Beta, TEL, PDGFRB, FLJ12858, PDGF2, SIS, SSV, C-sis, CD140B, JTK12, PDGFR, PDGFR1 Applications ELISA Indirect , FC , IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human PDGF Rβ (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831564 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing PDGF Rβ. A suggested method would be to stain cells expressing PDGF Rβ with 2.5 µg per 1-2.5 x 106 cells in a total staining volume of 200 µl followed by PN:G641. Western Blotting: To detect Human PDGF Rβ this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human PDGF Rβ is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P254. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: The ND50 for anti-human PDGF Rβ antibody was determined to be approximately 0.5-1.5 μg/ml for blocking 120 ng/ml recombinant human PDGF sRβ in the presence of 4 ng/ml of human PDGF-BB on a quiescent confluent culture of NR6R-3T3 cells. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Platelet-Derived Growth Factor Receptor Beta (PDGF Rβ) recognizes Human PDGF Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Platelet-derived growth factor receptor-β (PDGFRβ) is a well-characterized plasma membrane receptor with endogeneous tyrosine kinase activity, which is autophosphorylated in response to binding of dimeric platelet-derived growth factor (PDGF) ligand.1 PDGFRβ mediates signaling important for the proliferation, migration, and survival of mesenchymal cells.2 PDGFRβ is found in all cell types 3 and is activated in breast carcinoma cells and indicate a possible role of the receptor in breast cancerogenesis.4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Gilliland, DG. et al. (1996) Proc Natl Acad Sci USA 93: 14845 2. Ronnstrand, L. et al. (1998) Biochim Biophys Acta 1378: F79 3. Fischer, B. et al. (1997) Acta Anatomica 159: 194 4. Valius, M. et al. (2005) Biologija 1: 61 Technical Protocols  IHC FFPE N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
