TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human soluble TNF Receptor II (sTNFRII) is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
>97% by SDS-PAGE and analyzed by silver stain.
<0.01 EU/µg as determined bythe LAL method
The biological activity of Mouse Tumor Necrosis Factor Receptor Type II is determined by its inhibitory effect of the TNF-Alpha mediated cytotoxicity in mouse L-929 cells. The expected ED50 for this effect (in the presence of 0.25 ng/ml of recombinant Mouse TNF-alpha) is 0.125 µg/ml.
Protein Accession No.
Amino Acid Sequence
vpaqvvlt pykpepgyec qisqeyydrk aqmccakcpp gqyvkhfcnk tsdtvcadce asmytqvwnq frtclscsss cttdqveira ctkqqnrvca ceagrycalk thsgscrqcm rlskcgpgfg vassrapngn vlckacapgt fsdttsstdv crphricsil aipgnastda vcapesptls aiprtlyvsq peptrsqpld qepgpsqtps iltslgstpi ieqstkgg
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human sTNF RII is Mr 25 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217
2. Schall, TJ. et al. (1990) Cell 61:361
3. Tartaglia, LA. et al. (1992) Immunol. Today 13:151
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.