Recombinant Human CD120b (TNFR2) (aa 24-206)

TNF RII, also known as TNFRSF1B, is a transmembrane glycoprotein and member to the TNF receptor superfamily (1). It is limited primarily to hematopoietic cells and cells of the immune system and is produced naturally as a soluble form (sTNF RII) in various biological fluids (2). The soluble TNF receptor can bind TNF with high affinity and function as a TNF antagonist (3, 4). TNF RII is strongly expressed at the cartilage–pannus junction and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (1, 5). High plasma levels of sTNF RII were associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors (5). TNF RII has also been implicated in diabetes (6).

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Pro24

Lyophilized

The predicted molecular weight of Recombinant Human TNF RII is Mr 20 kDa.

20

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Dieudé, P. et al. (2002) Arthritis Care & Res. 46:2039
2. Ziegenhagen, MW. et al. (2000) J. Int. Med. 248:33
3. Chen, G. et al. (2002) Science 296:1634
4. Chung, A. et al. (2005) Mol. and Cell Biol. 25:4716
5. Wassink, TH. et al. (2000) Mol. Psych. 5:678
6. Shai, I. et al. (2005) Diabetes Care 28:1376