Anti-Rat IL-1 Rrp2
Anti-Rat IL-1 Rrp2
Product No.: I-680
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Clone 131011 Target IL-1 Rrp2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-1 R6, Interleukin 1 Receptor Related Protein 2 Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen NS0-Derived Recombinant Rat Interleukin-1 Receptor rp2 Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830801 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat IL-1 Rrp2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat IL-1 Rrp2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Rat Interlekin-1 Receptor rp2 (IL-1 Rrp2) (Clone 131011) recognizes an epitope on Rat IL-1 Rrp2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity rhIL-1 Rrp2 and no cross-reactivity with rmIL-1 RI, rmIL-1 RII, rhIL-1 RAcP, rmIL-1 R5, rmIL-1 R7 or rmIL-1 RAPL1. Background Interleukin-1 receptor related protein 2 (IL-1 Rrp2) is a receptor for IL-1F6 which is a proinflammatory member of IL-1 family. IL-1F8, and IL-1F9 also signal through IL-1Rrp2 and IL-1RAcP.1 IL-1F6, IL-1F8 and Il-1F9 are agonists and, along with their receptor IL-1Rrp2, are highly expressed in epithelial cells suggesting a role in immune defense in the skin and the gastrointestinal (GI) tract including the mouth. Synovial fibroblasts and articular chondrocytes also express IL-1Rrp2 and respond to IL-1F8, indicating a possible role in rheumatoid arthritis (RA).2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Sims, JE. et al. (2004) J Biol Chem. 279: 13677 2. Taylor, JJ. et al. (2007) Clin Exp Immunol. 147: 217 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
