Anti-Rat IL-1 Rrp2

Product No.: I-680

[product_table name="All Top" skus="I-680"]

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Clone
131011
Target
IL-1 Rrp2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-1 R6, Interleukin 1 Receptor Related Protein 2
Isotype
IgG1
Applications
WB

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Rat Interleukin-1 Receptor rp2
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat IL-1 Rrp2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat IL-1 Rrp2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Rat Interlekin-1 Receptor rp2 (IL-1 Rrp2) (Clone 131011) recognizes an epitope on Rat IL-1 Rrp2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity rhIL-1 Rrp2 and no cross-reactivity with rmIL-1 RI, rmIL-1 RII, rhIL-1 RAcP, rmIL-1 R5, rmIL-1 R7 or rmIL-1 RAPL1.
Background
Interleukin-1 receptor related protein 2 (IL-1 Rrp2) is a receptor for IL-1F6 which is a proinflammatory member of IL-1 family. IL-1F8, and IL-1F9 also signal through IL-1Rrp2 and IL-1RAcP.1 IL-1F6, IL-1F8 and Il-1F9 are agonists and, along with their receptor IL-1Rrp2, are highly expressed in epithelial cells suggesting a role in immune defense in the skin and the gastrointestinal (GI) tract including the mouth. Synovial fibroblasts and articular chondrocytes also express IL-1Rrp2 and respond to IL-1F8, indicating a possible role in rheumatoid arthritis (RA).2

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Sims, JE. et al. (2004) J Biol Chem. 279: 13677
2. Taylor, JJ. et al. (2007) Clin Exp Immunol. 147: 217
General Western Blot Protocol

Certificate of Analysis

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Prod No.
Description
I-680
I-593

Formats Available

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Prod No.
Description
I-680
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.