Anti-Mouse EphA6
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0 - Derived Recombinant Mouse EphA6 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830036 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse EphA6 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rmEphA6 is approximately 10 ng/lane and 5 ng/lane under non-reducingand reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse EphA6 recognizes Mouse EphA6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Ephrin receptor (EphA6) is a receptor1 that is the same as EHK2 (EPH homology kinase-2)2 which is expressed almost exclusively in nervous system. The receptor is expressed in cochlear ganglion cells.1 EphA6 is involved in neural circuits underlying aspects of learning and memory.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Lee, AM. et al. (1996) DNA and Cell Biol. 15: 817 2. Maisonpierre, PC. et al. (1997) Oncogene 8: 32777 3. Lanthorn, TH. et al. (2008) Neurosci Lett. 438: 205 Technical Protocols Certificate of Analysis |
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